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验证数据展示

  • IP experiment of mouse testis using 25822-1-AP

    IP experiment of mouse testis using 25822-1-AP

    IP result of anti-CCDC33 (IP:25822-1-AP, 4ug; Detection:25822-1-AP 1:600) with mouse testis tissue lysate 1800 ug.

  • WB analysis using 25822-1-AP

    WB analysis using 25822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25822-1-AP (CCDC33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CCDC33 Polyclonal antibody

CCDC33 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25822-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Cancer/testis antigen 61, CT61

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25822-1-AP targets CCDC33 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCDC33 fusion protein Ag22893 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 33
别名 Cancer/testis antigen 61, CT61
计算分子量 958 aa, 107 kDa
观测分子量85 kDa
GenBank蛋白编号BC025689
基因名称 CCDC33
Gene ID (NCBI) 80125
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5R6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human CCDC33 gene was reported to encode a cancer/testis (CT) antigen, which is located on 15q24.1, spans a region of 99.8 kb, and consists of 20 exons (PMID: 28639886). CCDC33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 5 different transcripts (37kda, 41kda, 85kda, 107kda, 114kda). Human CCDC33 was reported to be a cancer/testis (CT) protein. Abundant expression of Ccdc33 is evident during mouse spermatogenesis (PMID: 33942254).

实验方案

Product Specific Protocols
WB protocol for CCDC33 antibody 25822-1-APDownload protocol
IP protocol for CCDC33 antibody 25822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols