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验证数据展示

  • IP experiment of mouse testis using 25822-1-AP

    IP experiment of mouse testis using 25822-1-AP

    IP result of anti-CCDC33 (IP:25822-1-AP, 4ug; Detection:25822-1-AP 1:600) with mouse testis tissue lysate 1800 ug.

  • WB analysis using 25822-1-AP

    WB analysis using 25822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25822-1-AP (CCDC33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CCDC33 Polyclonal antibody

CCDC33 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25822-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Cancer/testis antigen 61, CT61

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25822-1-AP targets CCDC33 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22893

Product name: Recombinant human CCDC33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-206 aa of BC025689

Sequence: SEMNNYRRAMQKMAEDILSLRRQASILEGENRILRSRLAQQEEEEGQGKASEAQNTVSMKQKLLLSELDMKKLRDRVQHLQNELIRKNDREKELLLLYQAQQPQAALLKQYQGKLQKMKALEETVRHQEKVIEKMERVLEDRLQDRSKPPPLNRQQGKPYTGFPMLSASGLPLGSMGENLPVELYSVLLAENAKLRTELDKNRHQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 33
别名 Cancer/testis antigen 61, CT61
计算分子量 958 aa, 107 kDa
观测分子量85 kDa
GenBank蛋白编号BC025689
基因名称 CCDC33
Gene ID (NCBI) 80125
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5R6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human CCDC33 gene was reported to encode a cancer/testis (CT) antigen, which is located on 15q24.1, spans a region of 99.8 kb, and consists of 20 exons (PMID: 28639886). CCDC33 is predominantly expressed in the testis and undergoes alternative splicing to produce at least 5 different transcripts (37kda, 41kda, 85kda, 107kda, 114kda). Human CCDC33 was reported to be a cancer/testis (CT) protein. Abundant expression of Ccdc33 is evident during mouse spermatogenesis (PMID: 33942254).

实验方案

Product Specific Protocols
WB protocol for CCDC33 antibody 25822-1-APDownload protocol
IP protocol for CCDC33 antibody 25822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols