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验证数据展示

  • WB analysis using 21351-1-AP (same clone as 21351-1-PBS)

    WB analysis using 21351-1-AP (same clone as 21351-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21351-1-AP (CCDC136 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    IP experiment of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    IP result of anti-CCDC136 (IP:21351-1-AP, 4ug; Detection:21351-1-AP 1:800) with mouse testis tissue lysate 1800 ug. This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    IHC staining of mouse testis using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)

    IHC staining of mouse stomach using 21351-1-AP (same clone as 21351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21351-1-PBS in a different storage buffer formulation.

CCDC136 Polyclonal antibody, PBS Only

CCDC136 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 21351-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21351-1-PBS targets CCDC136 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15737

Product name: Recombinant human CCDC136 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 461-810 aa of BC109220

Sequence: AELQHLRDTVASFKESNEKDTETHAQLQEMKQLYQASKDELERQKHMYDQLEQDLLLCQLELKELKASHPIPEDKGKCANKCDTLLSRLTELQEKYKASQKEMGQLQMEQCELLEDQRRMQEEQGQLQEELHRLTLPLPKSGLLLKSQELLTKLEDLCELQLLYQGMQEEQKKLIQNQDCVLKEQLEIHEELRRFKESHFQEVLENPDDSKLAKSSKCNRNKQSKLLMEQMQALQVMYDAGQAKQELLQQEQGRLLEERKRLQADLQLCLEEMQLLQVQSPSIKMSLESYGKSYGSMVPSNENCRKTYDTTVDDNESYYKSYTSTQTSSKSFLKSYDSSTSASEAYGKSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 136
别名 Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein
计算分子量 1154 aa, 134 kDa
观测分子量110 kDa, 134 kDa
GenBank蛋白编号BC109220
基因名称 CCDC136
Gene ID (NCBI) 64753
RRIDAB_3742029
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JN2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC136 (also known as NAG6) is a coiled-coil domain containing protein described as a potential tumor suppressor in gastric cancer. CCDC136 protein was most abundantly located in the acrosome of round spermatids and elongating spermatids within seminiferous tubules of the adult mouse testis (PMID: 27076447).

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