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验证数据展示

  • WB analysis using 21351-1-AP

    WB analysis using 21351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21351-1-AP (CCDC136 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 21351-1-AP

    IP experiment of mouse testis using 21351-1-AP

    IP result of anti-CCDC136 (IP:21351-1-AP, 4ug; Detection:21351-1-AP 1:800) with mouse testis tissue lysate 1800 ug.

  • IHC staining of mouse testis using 21351-1-AP

    IHC staining of mouse testis using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 21351-1-AP

    IHC staining of mouse testis using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 21351-1-AP

    IHC staining of mouse stomach using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC136 Polyclonal antibody

CCDC136 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 21351-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21351-1-AP targets CCDC136 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15737

Product name: Recombinant human CCDC136 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 461-810 aa of BC109220

Sequence: AELQHLRDTVASFKESNEKDTETHAQLQEMKQLYQASKDELERQKHMYDQLEQDLLLCQLELKELKASHPIPEDKGKCANKCDTLLSRLTELQEKYKASQKEMGQLQMEQCELLEDQRRMQEEQGQLQEELHRLTLPLPKSGLLLKSQELLTKLEDLCELQLLYQGMQEEQKKLIQNQDCVLKEQLEIHEELRRFKESHFQEVLENPDDSKLAKSSKCNRNKQSKLLMEQMQALQVMYDAGQAKQELLQQEQGRLLEERKRLQADLQLCLEEMQLLQVQSPSIKMSLESYGKSYGSMVPSNENCRKTYDTTVDDNESYYKSYTSTQTSSKSFLKSYDSSTSASEAYGKSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 136
别名 Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein
计算分子量 1154 aa, 134 kDa
观测分子量110 kDa, 134 kDa
GenBank蛋白编号BC109220
基因名称 CCDC136
Gene ID (NCBI) 64753
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC136 (also known as NAG6) is a coiled-coil domain containing protein described as a potential tumor suppressor in gastric cancer. CCDC136 protein was most abundantly located in the acrosome of round spermatids and elongating spermatids within seminiferous tubules of the adult mouse testis (PMID: 27076447).

实验方案

Product Specific Protocols
IHC protocol for CCDC136 antibody 21351-1-APDownload protocol
IP protocol for CCDC136 antibody 21351-1-APDownload protocol
WB protocol for CCDC136 antibody 21351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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