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验证数据展示

  • WB analysis using 21351-1-AP

    WB analysis using 21351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21351-1-AP (CCDC136 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 21351-1-AP

    IP experiment of mouse testis using 21351-1-AP

    IP result of anti-CCDC136 (IP:21351-1-AP, 4ug; Detection:21351-1-AP 1:800) with mouse testis tissue lysate 1800 ug.

  • IHC staining of mouse testis using 21351-1-AP

    IHC staining of mouse testis using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 21351-1-AP

    IHC staining of mouse testis using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 21351-1-AP

    IHC staining of mouse stomach using 21351-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21351-1-AP (CCDC136 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC136 Polyclonal antibody

CCDC136 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 21351-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21351-1-AP targets CCDC136 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 CCDC136 fusion protein Ag15737 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 136
别名 Coiled-coil domain-containing protein 136, KIAA1793, NAG6, Nasopharyngeal carcinoma-associated gene 6 protein
计算分子量 1154 aa, 134 kDa
观测分子量110 kDa, 134 kDa
GenBank蛋白编号BC109220
基因名称 CCDC136
Gene ID (NCBI) 64753
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC136 (also known as NAG6) is a coiled-coil domain containing protein described as a potential tumor suppressor in gastric cancer. CCDC136 protein was most abundantly located in the acrosome of round spermatids and elongating spermatids within seminiferous tubules of the adult mouse testis (PMID: 27076447).

实验方案

Product Specific Protocols
WB protocol for CCDC136 antibody 21351-1-APDownload protocol
IHC protocol for CCDC136 antibody 21351-1-APDownload protocol
IP protocol for CCDC136 antibody 21351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols