• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 22041-1-AP

    WB analysis of Jurkat using 22041-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22041-1-AP (CCDC120 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 22041-1-AP

    IHC staining of human colon using 22041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 22041-1-AP

    IHC staining of human colon using 22041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

CCDC120 Polyclonal antibody

CCDC120 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22041-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (2)

别名

Coiled-coil domain-containing protein 120, JM11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, mouse liver tissue
Positive IHC detected inhuman colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22041-1-AP targets CCDC120 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CCDC120 fusion protein Ag14319 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 120
别名 Coiled-coil domain-containing protein 120, JM11
计算分子量 630 aa, 68 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC008769
基因名称 CCDC120
Gene ID (NCBI) 90060
RRIDAB_11182175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC120, also named as JM11, is required for neurite growth. In differentiating neuroblastoma cells, CCDC120 colocalizes with CYTH2 in both neurite shaft and growth cone areas. CCDC120 has some isoforms with MW 67-70 kDa.

实验方案

Product Specific Protocols
WB protocol for CCDC120 antibody 22041-1-APDownload protocol
IHC protocol for CCDC120 antibody 22041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.

Authors - Ning Huang
View Article
mouseWB,IF

J Biol Chem

Arf6 guanine-nucleotide exchange factor cytohesin-2 binds to CCDC120 and is transported along neurites to mediate neurite growth.

Authors - Tomohiro Torii
  • KD Validated
View Article