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  • Featured Product
  • KD/KO Validated

CCDC120 Polyclonal antibody

CCDC120 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 22041-1-AP

Print datasheet

Synonyms

CCDC120, JM11

验证数据展示

  • WB analysis of Jurkat using 22041-1-AP

    WB analysis of Jurkat using 22041-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22041-1-AP (CCDC120 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 22041-1-AP

    IHC staining of human colon using 22041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 22041-1-AP

    IHC staining of human colon using 22041-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 22041-1-AP (CCDC120 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, mouse liver tissue
Positive IHC detected inhuman colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22041-1-AP is CCDC120 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CCDC120 fusion protein Ag14319 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coiled-coil domain containing 120
Synonyms CCDC120, JM11
Calculated Molecular Weight 630 aa, 68 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC008769
Gene Symbol CCDC120
Gene ID (NCBI) 90060
RRIDAB_11182175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC120, also named as JM11, is required for neurite growth. In differentiating neuroblastoma cells, CCDC120 colocalizes with CYTH2 in both neurite shaft and growth cone areas. CCDC120 has some isoforms with MW 67-70 kDa.

实验方案

Product Specific Protocols
WB protocol for CCDC120 antibody 22041-1-APDownload protocol
IHC protocol for CCDC120 antibody 22041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.

Authors - Ning Huang
View Article
mouseWB,IF

J Biol Chem

Arf6 guanine-nucleotide exchange factor cytohesin-2 binds to CCDC120 and is transported along neurites to mediate neurite growth.

Authors - Tomohiro Torii
  • KD Validated
View Article