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验证数据展示

  • IP experiment of MOLT-4 using 31675-1-AP (same clone as 31675-1-PBS)

    IP experiment of MOLT-4 using 31675-1-AP (same clone as 31675-1-PBS)

    IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with MOLT-4 cells lysate 1800 ug. This data was developed using the same antibody clone with 31675-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 31675-1-AP (same clone as 31675-1-PBS)

    IP experiment of SH-SY5Y using 31675-1-AP (same clone as 31675-1-PBS)

    IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug. This data was developed using the same antibody clone with 31675-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31675-1-AP (same clone as 31675-1-PBS)

    IF Staining of A431 using 31675-1-AP (same clone as 31675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC102B antibody (31675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31675-1-PBS in a different storage buffer formulation.

CCDC102B Polyclonal antibody, PBS Only

CCDC102B Polyclonal Antibody for IF/ICC, IP, Indirect ELISA
Cat No. 31675-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, Indirect ELISA

别名

ACY1L, C18orf14

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31675-1-PBS targets CCDC102B in IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36379

Product name: Recombinant human CCDC102B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC126448

Sequence: MKPNLDGVDLFNNGGSGNGETKTGLRLKAINLPLENEVTEISALQVHLDEFQKILWKEREMRTALEKEIERLESALSLWKWKYEELKESKPKNVKEFDILLGQHNDEMQELSGNIKEESKSQNSKDRVICELRAELERLQAENTSEWDKREILEREKQGLERENRRLKIQVKEMEELLDKKNRLSANSQSPDFKMSQIDLQEKNQELLNLQHAYYKLNRQYQANIAELTHANNRVDQNEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 102B
别名 ACY1L, C18orf14
观测分子量60~68 kDa
GenBank蛋白编号BC126448
基因名称 CCDC102B
Gene ID (NCBI) 79839
RRIDAB_3742462
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ68D86
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835).

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