• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IP experiment of MOLT-4 using 31675-1-AP

    IP experiment of MOLT-4 using 31675-1-AP

    IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with MOLT-4 cells lysate 1800 ug.

  • IP experiment of SH-SY5Y using 31675-1-AP

    IP experiment of SH-SY5Y using 31675-1-AP

    IP result of anti-CCDC102B (IP:31675-1-AP, 4ug; Detection:31675-1-AP 1:800) with SH-SY5Y cells lysate 1240 ug.

  • IF Staining of A431 using 31675-1-AP

    IF Staining of A431 using 31675-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC102B antibody (31675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CCDC102B Polyclonal antibody

CCDC102B Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 31675-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

ACY1L, C18orf14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IP detected inSH-SY5Y cells, MOLT-4 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31675-1-AP targets CCDC102B in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36379

Product name: Recombinant human CCDC102B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC126448

Sequence: MKPNLDGVDLFNNGGSGNGETKTGLRLKAINLPLENEVTEISALQVHLDEFQKILWKEREMRTALEKEIERLESALSLWKWKYEELKESKPKNVKEFDILLGQHNDEMQELSGNIKEESKSQNSKDRVICELRAELERLQAENTSEWDKREILEREKQGLERENRRLKIQVKEMEELLDKKNRLSANSQSPDFKMSQIDLQEKNQELLNLQHAYYKLNRQYQANIAELTHANNRVDQNEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 102B
别名 ACY1L, C18orf14
观测分子量60~68 kDa
GenBank蛋白编号BC126448
基因名称 CCDC102B
Gene ID (NCBI) 79839
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ68D86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled coil domain containing 102B (CCDC102B) is a new centrosome-linker protein that is required for maintaining centrosome cohesion. Human CCDC102B is an uncharacterized protein containing 513 amino acids (aa); it is predicted to contain one short coiled-coil domain on its N-terminus and two long coiled-coil domains in the middle region and C-terminus, respectively (PMID: 35957886). At the onset of mitosis, along with other centrosome components, CCDC102B is phosphorylated by Nek2A and is disassociated from the centrosome to allow for the centrosome separation (PMID: 30404835).

实验方案

Product Specific Protocols
IF protocol for CCDC102B antibody 31675-1-APDownload protocol
IP protocol for CCDC102B antibody 31675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...