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验证数据展示

  • WB analysis of HeLa using 24061-1-AP

    WB analysis of HeLa using 24061-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24061-1-AP (SGF29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24061-1-AP

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 10x lens).

  • IHC staining of human liver cancer using 24061-1-AP

    IHC staining of human liver cancer using 24061-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24061-1-AP (SGF29 antibody at dilution of 1:400 (under 40x lens).

  • Featured Product
  • KD/KO验证

SGF29 Polyclonal antibody

SGF29 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24061-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CCDC101, SGF29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24061-1-AP targets SGF29 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SGF29 fusion protein Ag21261 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 101
别名 CCDC101, SGF29
计算分子量 293 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC011981
基因名称 SGF29
Gene ID (NCBI) 112869
RRIDAB_2879420
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96ES7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

实验方案

Product Specific Protocols
WB protocol for SGF29 antibody 24061-1-APDownload protocol
IHC protocol for SGF29 antibody 24061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
  • KO Validated
View Article