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验证数据展示

  • WB analysis using 55476-1-AP

    WB analysis using 55476-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55476-1-AP (CCBL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 55476-1-AP

    IF Staining of K-562 using 55476-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CCBL2 antibody (55476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCBL2 Polyclonal antibody

CCBL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55476-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RBMXL1, KATIII, KAT3, GTL, Glutamine Transaminase L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55476-1-AP targets CCBL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine conjugate-beta lyase 2
别名 RBMXL1, KATIII, KAT3, GTL, Glutamine Transaminase L
计算分子量 51 kDa
观测分子量48 kDa，51- 55 kda
GenBank蛋白编号NM_019610
基因名称 CCBL2
Gene ID (NCBI) 56267
RRIDAB_3670254
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YP21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cysteine conjugate beta-lyase 2 (CCBL2), also known as kynurenine aminotransferase 3 (KAT3) or glutamine transaminase L (GTL), can effectively catalyze the transamination of glutamine, methionine, and kynurenine (KYN) to kynurenic acid (KYNA) as well as the pathway of drug metabolism by cytochrome P450. CCBL2 has been found in mouse, rat, and human whose mRNA is widely expressed in several organs such as the liver, kidney, heart, and neuroendocrine tissues (PMID: 35771747).

实验方案

Product Specific Protocols
WB protocol for CCBL2 antibody 55476-1-APDownload protocol
IF protocol for CCBL2 antibody 55476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols