CBY1 Polyclonal antibody
CBY1 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12239-1-AP targets CBY1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CBY1 fusion protein Ag2882 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chibby homolog 1 (Drosophila) |
| Synonyms | arb1, ARPP binding protein, C22orf2, CBY, CBY1, chibby homolog 1 (Drosophila), Cytosolic leucine rich protein, HS508I15A, PGEA1, PIGEA 14, PIGEA14, Protein chibby homolog 1 |
| Calculated Molecular Weight | 126 aa, 14 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC016139 |
| Gene Symbol | CBY1 |
| Gene ID (NCBI) | 25776 |
| RRID | AB_10951858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3M2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBY1 antibody 12239-1-AP | Download protocol |
| IHC protocol for CBY1 antibody 12239-1-AP | Download protocol |
| IP protocol for CBY1 antibody 12239-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. | ||
Dev Dyn DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism. | ||
Medicine (Baltimore) Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma |
