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验证数据展示

  • WB analysis using 83248-4-RR (same clone as 83248-4-PBS)

    WB analysis using 83248-4-RR (same clone as 83248-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83248-4-RR (CBX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 83248-4-RR (same clone as 83248-4-PBS)

    WB analysis of MCF-7 using 83248-4-RR (same clone as 83248-4-PBS)

    WB result of CBX1 antibody (83248-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    IF Staining of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-4-RR, Clone: 240075H6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    FC experiment of HeLa using 83248-4-RR (same clone as 83248-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cbx1 Recombinant Antibody (83248-4-RR, Clone:240075H6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83248-4-RR

    Affinity and Kinetic Characterization of 83248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83248-4-RR against Human CBX1 were performed. The affinity constant is 0.605 nM.

  • Featured Product
  • KD/KO验证

CBX1 Recombinant monoclonal antibody, PBS Only

CBX1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-4-PBS

产品说明书

CloneNo. 240075H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240075H6, CBX, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

产品信息

83248-4-PBS targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34901

Product name: Recombinant human CBX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-115 aa of BC002609

Sequence: CPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromobox homolog 1 (HP1 beta homolog Drosophila )
别名 240075H6, CBX, Chromobox protein homolog 1, Heterochromatin protein p25, HP1 beta
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC002609
基因名称 CBX1
Gene ID (NCBI) 10951
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP83916
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

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