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验证数据展示

  • WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

    WB analysis using 83212-4-RR (same clone as 83212-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83212-4-RR (same clone as 83212-4-PBS)

    WB analysis of HEK-293 using 83212-4-RR (same clone as 83212-4-PBS)

    WB result of NCBP1/CBP80 antibody (83212-4-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCBP1/CBP80 transfected HEK-293 cells. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83212-4-RR (same clone as 83212-4-PBS)

    IHC staining of human ovary cancer using 83212-4-RR (same clone as 83212-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCBP1 antibody included
  • FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    FC experiment of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    IF Staining of HepG2 using 83212-4-RR (same clone as 83212-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83212-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83212-4-RR

    Affinity and Kinetic Characterization of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

  • Featured Product
  • KD/KO验证

NCBP1/CBP80 Recombinant monoclonal antibody, PBS Only

NCBP1/CBP80 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83212-4-PBS

产品说明书

CloneNo. 240012G8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

NCBP1, 240012G8, 80 kDa nuclear cap-binding protein, CBP80, NCBP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCBP1 Ready-To-Use IHC Kit. NCBP1 primary antibody included.

产品信息

83212-4-PBS targets NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag0496

Product name: Recombinant human CBP80 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 46-350 aa of BC001450

Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear cap binding protein subunit 1, 80kDa
别名 NCBP1, 240012G8, 80 kDa nuclear cap-binding protein, CBP80, NCBP
计算分子量 92 kDa
观测分子量 80 kDa
GenBank蛋白编号BC001450
基因名称 NCBP1
Gene ID (NCBI) 4686
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ09161
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

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