CBLB Polyclonal antibody
CBLB Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse colon tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12781-1-AP targets CBLB in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, FC, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CBLB fusion protein Ag3491 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence b |
| Synonyms | CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B |
| Calculated Molecular Weight | 982 aa, 109 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC032851 |
| Gene Symbol | CBLB |
| Gene ID (NCBI) | 868 |
| RRID | AB_2275326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13191 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CBLB(E3 ubiquitin-protein ligase CBL-B) is also named as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBLB antibody 12781-1-AP | Download protocol |
| IHC protocol for CBLB antibody 12781-1-AP | Download protocol |
| IF protocol for CBLB antibody 12781-1-AP | Download protocol |
| IP protocol for CBLB antibody 12781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. | ||
J Immunol A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function. | ||
J Cell Mol Med NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection. | ||
Biomolecules miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles. |
