验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32840-1-AP targets CATSPERE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag36791 Product name: Recombinant human C1orf101 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 600-804 aa of BC032859 Sequence: DKGEALTVWTQIVYPENTGLYVIVESYGPKILQESHEISFEAAFGYCTKTLTLTFYQNVDYERISDYFETQDKHTGLVLVQFRPSEYSKACPIAQKVFQIAVGCDDKKFIAIKGFSKKGCHHHDFSYVIEKSYLRHQPSKNLRVRYIWGEYGCPLRLDFTEKFQPVVQLFDDNGYVKDVEANFIVWEIHGRDDYSFNNTMAQSGC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 1 open reading frame 101 |
| 别名 | C1orf101, Cation channel sperm-associated auxiliary subunit epsilon, CatSperepsilon, CatSper-epsilon |
| 计算分子量 | 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC032859 |
| 基因名称 | C1orf101 |
| Gene ID (NCBI) | 257044 |
| RRID | AB_3742759 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5SY80 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cation channel sperm-associated auxiliary subunit epsilon (CATSPERE) is a critical component of the CatSper calcium channel complex, essential for sperm function and male fertility. CATSPERE interacts with the pore-forming subunits and contributes to the stability and function of the entire CatSper complex (PMID: 39605618). This complex is crucial for sperm capacitation, which includes the development of hyperactivated motility and the acrosome reaction, necessary for successful fertilization (PMID: 17330112; 18508611; 29707693).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CATSPERE antibody 32840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
