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验证数据展示

  • IF Staining of C2C12 using 84056-6-RR

    IF Staining of C2C12 using 84056-6-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of C2C12 using 84056-6-RR

    IF Staining of C2C12 using 84056-6-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of U2OS using 84056-6-RR

    FC experiment of U2OS using 84056-6-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 84056-6-RR

    WB analysis using 84056-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84056-6-RR (CASQ2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 84056-6-RR

    IHC staining of mouse skeletal muscle using 84056-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 84056-6-RR

    IHC staining of mouse skeletal muscle using 84056-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84056-6-RR

    Affinity and Kinetic Characterization of 84056-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84056-6-RR against Human CASQ2 were performed. The affinity constant is 0.251 nM.

Calsequestrin 2 Recombinant monoclonal antibody

Calsequestrin 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84056-6-RR

产品说明书

CloneNo. 241211A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

CASQ2, 241211A3, Calsequestrin-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhuman heart tissue, mouse heart tissue, rat heart tissue, pig heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inU2OS cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84056-6-RR targets Calsequestrin 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag33584

Product name: Recombinant human CASQ2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-399 aa of BC022288

Sequence: MKRTHLFIVGIYFLSSCRAEEGLNFPTYDGKDRVVSLSEKNFKQVLKKYDLLCLYYHEPVSSDKVTQKQFQLKEIVLELVAQVLEHKAIGFVMVDAKKEAKLAKKLGFDEEGSLYILKGDRTIEFDGEFAADVLVEFLLDLIEDPVEIISSKLEVQAFERIEDYIKLIGFFKSEDSEYYKAFEEAAEHFQPYIKFFATFDKGVAKKLSLKMNEVDFYEPFMDEPIAIPNKPYTEEELVEFVKEHQRPTLRRLRPEEMFETWEDDLNGIHIVAFAEKSDPDGYEFLEILKQVARDNTDNPDLSILWIDPDDFPLLVAYWEKTFKIDLFRPQIGVVNVTDADSVWMEIPDDDDLPTAEELEDWIEDVLSGKINTEDDDEDDDDDDNSDEEDNDDSDDDDDE*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calsequestrin 2 (cardiac muscle)
别名 CASQ2, 241211A3, Calsequestrin-2
计算分子量 46 kDa
观测分子量 46-55 kDa
GenBank蛋白编号BC022288
基因名称 Calsequestrin 2
Gene ID (NCBI) 845
RRIDAB_3671624
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO14958
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

实验方案

Product Specific Protocols
IF protocol for Calsequestrin 2 antibody 84056-6-RRDownload protocol
IHC protocol for Calsequestrin 2 antibody 84056-6-RRDownload protocol
WB protocol for Calsequestrin 2 antibody 84056-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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