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验证数据展示

  • IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

    IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

    IF Staining of C2C12 using 84056-6-RR (same clone as 84056-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CASQ2 antibody (84056-6-RR, Clone: 241211A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84056-6-RR (same clone as 84056-6-PBS)

    FC experiment of U2OS using 84056-6-RR (same clone as 84056-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CASQ2 Recombinant antibody (84056-6-RR, Clone:241211A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • WB analysis using 84056-6-RR (same clone as 84056-6-PBS)

    WB analysis using 84056-6-RR (same clone as 84056-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84056-6-RR (CASQ2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

    IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:0 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

    IHC staining of mouse skeletal muscle using 84056-6-RR (same clone as 84056-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84056-6-RR (CASQ2 antibody) at dilution of 1:0 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84056-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84056-6-RR

    Affinity and Kinetic Characterization of 84056-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84056-6-RR against Human CASQ2 were performed. The affinity constant is 0.251 nM.

Calsequestrin 2 Recombinant monoclonal antibody, PBS Only

Calsequestrin 2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84056-6-PBS

产品说明书

CloneNo. 241211A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

CASQ2, 241211A3, Calsequestrin-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84056-6-PBS targets Calsequestrin 2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag33584

Product name: Recombinant human CASQ2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-399 aa of BC022288

Sequence: MKRTHLFIVGIYFLSSCRAEEGLNFPTYDGKDRVVSLSEKNFKQVLKKYDLLCLYYHEPVSSDKVTQKQFQLKEIVLELVAQVLEHKAIGFVMVDAKKEAKLAKKLGFDEEGSLYILKGDRTIEFDGEFAADVLVEFLLDLIEDPVEIISSKLEVQAFERIEDYIKLIGFFKSEDSEYYKAFEEAAEHFQPYIKFFATFDKGVAKKLSLKMNEVDFYEPFMDEPIAIPNKPYTEEELVEFVKEHQRPTLRRLRPEEMFETWEDDLNGIHIVAFAEKSDPDGYEFLEILKQVARDNTDNPDLSILWIDPDDFPLLVAYWEKTFKIDLFRPQIGVVNVTDADSVWMEIPDDDDLPTAEELEDWIEDVLSGKINTEDDDEDDDDDDNSDEEDNDDSDDDDDE*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calsequestrin 2 (cardiac muscle)
别名 CASQ2, 241211A3, Calsequestrin-2
计算分子量 46 kDa
观测分子量 46-55 kDa
GenBank蛋白编号BC022288
基因名称 Calsequestrin 2
Gene ID (NCBI) 845
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO14958
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

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