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验证数据展示

  • WB analysis of SK-BR-3 using 33597-1-AP (same clone as 33597-1-PBS)

    WB analysis of SK-BR-3 using 33597-1-AP (same clone as 33597-1-PBS)

    WB result of CARS2 antibody (33597-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARS2 transfected SK-BR-3 cells. This data was developed using the same antibody clone with 33597-1-PBS in a different storage buffer formulation.

  • IP experiment of SK-BR-3 using 33597-1-AP (same clone as 33597-1-PBS)

    IP experiment of SK-BR-3 using 33597-1-AP (same clone as 33597-1-PBS)

    IP result of anti-CARS2 (IP:33597-1-AP, 4ug; Detection:33597-1-AP 1:3000) with SK-BR-3 cells lysate 1600 ug. This data was developed using the same antibody clone with 33597-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 33597-1-AP (same clone as 33597-1-PBS)

    IF Staining of A431 using 33597-1-AP (same clone as 33597-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CARS2 antibody (33597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33597-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CARS2 Polyclonal antibody, PBS Only

CARS2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 33597-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CysRS, Cysteinyl-tRNA synthetase, EC:6.1.1.16, OK/SW-cl.10, Probable cysteine--tRNA ligase, mitochondrial

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33597-1-PBS targets CARS2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38943

Product name: Recombinant human CARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-564 aa of NM_024537.3

Sequence: FLKTFSPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKGQLACGSVREAMLWERLSSTKRAVKAALADDFDTPRVVDAILGLAHHGNGQLRASLKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQFALAMPEATGDARRQQLLERQPLLEACDTLRRGLTAHGINIKDRSSTTSTWELLDQRTKDQKSAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteinyl-tRNA synthetase 2, mitochondrial (putative)
别名 CysRS, Cysteinyl-tRNA synthetase, EC:6.1.1.16, OK/SW-cl.10, Probable cysteine--tRNA ligase, mitochondrial
计算分子量62kDa，564aa
观测分子量55-63kd
GenBank蛋白编号NM_024537.3
基因名称 CARS2
Gene ID (NCBI) 79587
RRIDAB_3743024
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HA77
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cars2 (mitochondrial cysteine-tRNA synthetase 2) is a class I aminoacyl-tRNA synthetase encoded by nuclear gene, which locates in mitochondria. Its core function is to catalyze cysteine to bind to corresponding trna, and ensure the translation of mitochondrial protein. Its mutation will cause diseases related to mitochondrial dysfunction.

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