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验证数据展示

  • WB analysis of SK-BR-3 using 33597-1-AP

    WB analysis of SK-BR-3 using 33597-1-AP

    WB result of CARS2 antibody (33597-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARS2 transfected SK-BR-3 cells.

  • IP experiment of SK-BR-3 using 33597-1-AP

    IP experiment of SK-BR-3 using 33597-1-AP

    IP result of anti-CARS2 (IP:33597-1-AP, 4ug; Detection:33597-1-AP 1:3000) with SK-BR-3 cells lysate 1600 ug.

  • IF Staining of A431 using 33597-1-AP

    IF Staining of A431 using 33597-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CARS2 antibody (33597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

CARS2 Polyclonal antibody

CARS2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 33597-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

CysRS, Cysteinyl-tRNA synthetase, EC:6.1.1.16, OK/SW-cl.10, Probable cysteine--tRNA ligase, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSK-BR-3 cells
Positive IP detected inSK-BR-3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33597-1-AP targets CARS2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38943

Product name: Recombinant human CARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-564 aa of NM_024537.3

Sequence: FLKTFSPDVFRFFCLRSSYRSAIDYSDSAMLQAQQLLLGLGSFLEDARAYMKGQLACGSVREAMLWERLSSTKRAVKAALADDFDTPRVVDAILGLAHHGNGQLRASLKEPEGPRSPAVFGAIISYFEQFFETVGISLANQQYVSGDGSEATLHGVVDELVRFRQKVRQFALAMPEATGDARRQQLLERQPLLEACDTLRRGLTAHGINIKDRSSTTSTWELLDQRTKDQKSAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteinyl-tRNA synthetase 2, mitochondrial (putative)
别名 CysRS, Cysteinyl-tRNA synthetase, EC:6.1.1.16, OK/SW-cl.10, Probable cysteine--tRNA ligase, mitochondrial
计算分子量62kDa，564aa
观测分子量55-63kd
GenBank蛋白编号NM_024537.3
基因名称 CARS2
Gene ID (NCBI) 79587
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HA77
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cars2 (mitochondrial cysteine-tRNA synthetase 2) is a class I aminoacyl-tRNA synthetase encoded by nuclear gene, which locates in mitochondria. Its core function is to catalyze cysteine to bind to corresponding trna, and ensure the translation of mitochondrial protein. Its mutation will cause diseases related to mitochondrial dysfunction.

实验方案

Product Specific Protocols
IF protocol for CARS2 antibody 33597-1-APDownload protocol
IP protocol for CARS2 antibody 33597-1-APDownload protocol
WB protocol for CARS2 antibody 33597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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