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验证数据展示

  • WB analysis using 68657-1-Ig

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 68657-1-Ig

    WB analysis of THP-1 using 68657-1-Ig

    WB result of CARD9 antibody (68657-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells.

  • IF Staining of HeLa using 68657-1-Ig

    IF Staining of HeLa using 68657-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  • KD/KO验证

CARD9 Monoclonal antibody

CARD9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68657-1-Ig

产品说明书

CloneNo. 2F3E10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNK-92 cells, HL-60 cells, TF-1 cells, THP-1 cells, U-937 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68657-1-Ig targets CARD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33643

Product name: Recombinant human CARD9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 193-492 aa of BC008877

Sequence: SEEKGAALMRNRDLQLEIDQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYIQVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQMEEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLETLVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGPAGLPGIGAVC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 9
别名 hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10
计算分子量 62 kDa
观测分子量55-65 kDa
GenBank蛋白编号BC008877
基因名称 CARD9
Gene ID (NCBI) 64170
RRIDAB_3670399
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H257
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

实验方案

Product Specific Protocols
IF protocol for CARD9 antibody 68657-1-IgDownload protocol
WB protocol for CARD9 antibody 68657-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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