验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
10400-1-AP targets CARD14 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0628 Product name: Recombinant human CARD14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC018142 Sequence: MGELCRRDSALTALDEETLWEMMESHRHRIVRCICPSRLTPYLRQAKVLCQLDEEEVLHSPRLTNSAMRAGHLLDLLKTRGKNGAIAFLESLKFHNPDVYTLVTGLQPDVDFSNFSGLMETSKLTECLAGAIGSLQEELNQEKGQKEVLLRRCQQLQEHLGLAETRAEGLHQLEADHSRMKREVSAHFHEVLRLKDEMLSLSLHYSNALQEKELAASRCRSLQEELYLLKQELQRANMVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFSLAEKDILEQSLDEARGSRQE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase recruitment domain family, member 14 |
| 别名 | BIMP2, CARD14, Carma 2, CARMA2 |
| 计算分子量 | 113 kDa |
| 观测分子量 | 113-140 kDa |
| GenBank蛋白编号 | BC018142 |
| 基因名称 | CARD14 |
| Gene ID (NCBI) | 79092 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CARD14/CARMA2 is a novel caspase recruitment domain-containing protein 14 and is also a membrane-associated guanylate kinase (MAGUK) family member. CARD domain is a protein-binding element that mediates the assembly of CARD-containing proteins into apoptosis and NF-kappaB signaling complexes, which has been implicated in inflammatory disorders. CARD14 activates NF-kappaB signaling via interaction with BCL10. Defects in CARD14 are the cause of susceptibility to psoriasis type 2 (PSORS2), which is a common inflammatory disorder of the skin and other organs caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene MALT1 is a critical mediator of PAR1-driven NF-κB activation and metastasis in multiple tumor types. | ||
EMBO Mol Med CARD14 signaling in intestinal epithelial cells induces intestinal inflammation and intestinal transit delay. | ||
Biochem Pharmacol Polo-like kinase 1 (PLK1) is a novel CARD14-binding protein in keratinocytes. | ||
Biomedicines CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells | ||
Biochem J CARD14 signalosome formation is associated with its endosomal relocation and mTORC1-induced keratinocyte proliferation |
