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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

CAPN2 Monoclonal antibody, PBS Only

CAPN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66977-1-PBS

产品说明书

CloneNo. 1E1F10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1E1F10, Calcium-activated neutral proteinase 2, Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAPN2 Ready-To-Use IHC Kit. CAPN2 primary antibody included.

产品信息

66977-1-PBS targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28427

Product name: Recombinant human CAPN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-330 aa of BC021303

Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calpain 2, (m/II) large subunit
别名 1E1F10, Calcium-activated neutral proteinase 2, Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit
计算分子量 700 aa, 80 kDa
观测分子量 72-80 kDa
GenBank蛋白编号BC021303
基因名称 CAPN2
Gene ID (NCBI) 824
RRIDAB_2882297
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP17655
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.