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KD/KO Validated

Calpain 2 Polyclonal antibody

Calpain 2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 11472-1-AP

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Synonyms

Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, M calpain, mCANP, Millimolar calpain

验证数据展示

WB analysis using 11472-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-231 using 11472-1-AP
MDA-MB-231 (WT and CAPN2 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 11472-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of HeLa using 11472-1-AP
WB result of Calpain 2 antibody (11472-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 2 transfected HeLa cells.
IHC staining of human colon cancer using 11472-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPN2 antibody included
IHC staining of human kidney using 11472-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CAPN2 antibody included
WB analysis using 11472-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of MDA-MB-231 using 11472-1-AP
MDA-MB-231 lysates prepared and IP of CAPN2 performed using 1.0 μg of 11472-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IF Staining of MDA-MB-231 using 11472-1-AP
MDA-MB-231 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11472-1-AP at 1:300, plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IF Staining of HepG2 using 11472-1-AP
Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
WB analysis using 11472-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Cat No. 11472-1-AP

Size

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经过测试的应用

Positive WB detected in	A549 cells, MDA-MB-231 cells, human placenta tissue, HeLa cells, Y79 cells, C2C12 cells, mouse brain tissue, rat brain tissue, K-562 cells, PC-3 cells
Positive IP detected in	MDA-MB-231 cells
Positive IHC detected in	human kidney tissue, human colon cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, MDA-MB-231 cells

Planning an IHC experiment? We recommend our IHCeasy CAPN2 Ready-To-Use IHC Kit. CAPN2 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 14 publications below
IHC	See 3 publications below
IF	See 4 publications below

产品信息

11472-1-AP targets Calpain 2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IF, IHC
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	Calpain 2 fusion protein Ag2029 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	calpain 2, (m/II) large subunit
Synonyms	Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, M calpain, mCANP, Millimolar calpain
Calculated Molecular Weight	700 aa, 80 kDa
Observed Molecular Weight	72-80 kDa
GenBank Accession Number	BC021303
Gene Symbol	CAPN2
Gene ID (NCBI)	824
RRID	AB_10755306
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17655
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

实验方案

Product Specific Protocols
WB protocol for Calpain 2 antibody 11472-1-AP	Download protocol
IHC protocol for Calpain 2 antibody 11472-1-AP	Download protocol
IF protocol for Calpain 2 antibody 11472-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IHC	Phytomedicine Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells Authors - Maolei Xu View Article
mouse	WB	Pharmacol Res Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Authors - Juan Wang View Article
mouse	IF	Pharmacol Res TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy. Authors - Wen Tian View Article
mouse	WB	J Am Heart Assoc Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction Authors - Kurt D Marshall View Article
rat	WB	Front Pharmacol Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway. Authors - Lei Liu View Article
Rat	WB,IHC	Int Immunopharmacol Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest. Authors - Wen-Yan Wang View Article

Calpain 2 Polyclonal antibody

Calpain 2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis using 11472-1-AP

WB analysis of MDA-MB-231 using 11472-1-AP

WB analysis of HeLa using 11472-1-AP

IHC staining of human colon cancer using 11472-1-AP

IHC staining of human kidney using 11472-1-AP

WB analysis using 11472-1-AP

IP experiment of MDA-MB-231 using 11472-1-AP

IF Staining of MDA-MB-231 using 11472-1-AP

IF Staining of HepG2 using 11472-1-AP

WB analysis using 11472-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction

Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.

Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.