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验证数据展示

  • WB analysis using 82720-14-RR (same clone as 82720-14-PBS)

    WB analysis using 82720-14-RR (same clone as 82720-14-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82720-14-RR (CAMKK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82720-14-PBS in a different storage buffer formulation.

  • WB analysis using 82720-14-RR (same clone as 82720-14-PBS)

    WB analysis using 82720-14-RR (same clone as 82720-14-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82720-14-RR (CAMKK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82720-14-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 82720-14-RR (same clone as 82720-14-PBS)

    IF Staining of PC-3 using 82720-14-RR (same clone as 82720-14-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (82720-14-RR, Clone: 251379E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82720-14-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82720-14-RR (same clone as 82720-14-PBS)

    IHC staining of human colon cancer using 82720-14-RR (same clone as 82720-14-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82720-14-RR (CAMKK2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82720-14-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMKK2 antibody included
  • Affinity and Kinetic Characterization of 82720-14-RR

    Affinity and Kinetic Characterization of 82720-14-RR

    Biolayer interferometry (BLl) kinetic assays of 82720-14-RR against Human CAMKK2 were performed. The affinity constant is below 1 pM

CAMKK2 Recombinant monoclonal antibody, PBS Only

CAMKK2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82720-14-PBS

产品说明书

CloneNo. 251379E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Calcium/calmodulin-dependent protein kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CAMKK2 Ready-To-Use IHC Kit. CAMKK2 primary antibody included.

产品信息

82720-14-PBS targets CAMKK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2093

Product name: Recombinant human CAMKK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC026060

Sequence: MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDSSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase kinase 2, beta
别名 Calcium/calmodulin-dependent protein kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta, CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2
计算分子量 588 aa, 60 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC026060
基因名称 CAMKK2
Gene ID (NCBI) 10645
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96RR4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

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