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验证数据展示

  • WB analysis of mouse brain using 55140-1-AP

    WB analysis of mouse brain using 55140-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CAMK2G antibody included
  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CAMK2G antibody included

CaMKII gamma Polyclonal antibody

CaMKII gamma Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55140-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CaMKII gamma-Specific, CAMK2G, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CAMK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAMK2G Ready-To-Use IHC Kit. CAMK2G primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55140-1-AP targets CaMKII gamma in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II gamma
别名 CaMKII gamma-Specific, CAMK2G, Calcium/calmodulin-dependent protein kinase type II subunit gamma, CaM kinase II subunit gamma, CAMK
计算分子量 65 kDa
观测分子量 45-65 kDa
GenBank蛋白编号NM_001222
基因名称 CAMK2G
Gene ID (NCBI) 818
RRIDAB_10858395
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CaMK2 is a Ca2+-signaling intermediate that contains α, β, γ and δ subunits.

实验方案

Product Specific Protocols
WB protocol for CaMKII gamma antibody 55140-1-APDownload protocol
IHC protocol for CaMKII gamma antibody 55140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
View Article
humanWB

Biomed Pharmacother

Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.

Authors - Qingfeng Yu
View Article