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CaMKII gamma-Specific Polyclonal antibody

CaMKII gamma-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 55140-1-AP

Print datasheet

Synonyms

CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma

验证数据展示

  • WB analysis of mouse brain using 55140-1-AP

    WB analysis of mouse brain using 55140-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55140-1-AP targets CaMKII gamma-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II gamma
Synonyms CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 45-65 kDa
GenBank Accession NumberNM_001222
Gene Symbol CAMK2G
Gene ID (NCBI) 818
RRIDAB_10858395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named as CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CaMK2 is a Ca2+-signaling intermediate that contains α, β, γ and δ subunits. The antibody is specific to CAMK2G.

实验方案

Product Specific Protocols
WB protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
IHC protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
View Article
humanWB

Biomed Pharmacother

Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.

Authors - Qingfeng Yu
View Article