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CAMK2B Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse, rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.625-20 ng/mL

Cat no : MP02919-1

Synonyms

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02919-1, Human CAMK2B Recombinant Matched Antibody Pair - PBS only. 86858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2119. 86858-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

    Sandwich ELISA standard curve of MP02919-1

    Sandwich ELISA standard curve of MP02919-1, Human CAMK2B Recombinant Matched Antibody Pair - PBS only. 86858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2119. 86858-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

  • The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

    Sandwich ELISA sample test of MP02919-1

    The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP02919-1 targets CAMK2B in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.625-20 ng/mL (Sandwich ELISA);

Capture antibody: CAMK2B Recombinant monoclonal antibody, PBS Only (Capture) 86858-2-PBS (251925E3). 100 μg. Concentration 1 mg/ml.

Detection antibody: CAMK2B Recombinant monoclonal antibody, PBS Only (Detector) 86858-1-PBS (251925D4). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag2119

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86858-2-PBS
Tested Reactivity human
Immunogen CAMK2B fusion protein Ag2119
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II beta
Synonyms
GenBank accession numberBC019070
Gene symbol CaMKII beta
UniprotQ13554
Gene ID (NCBI) 816
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86858-1-PBS
Tested Reactivity human, mouse, rat
Immunogen CAMK2B fusion protein Ag2119
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II beta
Synonyms Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2
GenBank accession numberBC019070
Gene symbol CaMKII beta
UniprotQ13554
Gene ID (NCBI) 816
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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