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验证数据展示

  • WB analysis of U-251 using 86858-1-RR (same clone as 86858-1-PBS)

    WB analysis of U-251 using 86858-1-RR (same clone as 86858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86858-1-RR (CAMK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86858-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02919-1

    Sandwich ELISA sample test of MP02919-1

    The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02919-1

    Sandwich ELISA standard curve of MP02919-1

    Sandwich ELISA standard curve of MP02919-1, Human CAMK2B Recombinant Matched Antibody Pair - PBS only. 86858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2119. 86858-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 86858-1-RR

    Affinity and Kinetic Characterization of 86858-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86858-1-RR against Human CAMK2B were performed. The affinity constant is below 1 pM.

CAMK2B Recombinant monoclonal antibody, PBS Only (Detector)

CAMK2B Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 86858-1-PBS

产品说明书

CloneNo. 251925D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Sandwich ELISA, Indirect ELISA

别名

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86858-1-PBS targets CAMK2B as part of a matched antibody pair:

MP02919-1: 86858-2-PBS capture and 86858-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2119

Product name: Recombinant human CAMK2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC019070

Sequence: MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II beta
别名 Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2
计算分子量 503 aa, 56 kDa
观测分子量50-70 kDa
GenBank蛋白编号BC019070
基因名称 CaMKII beta
Gene ID (NCBI) 816
RRIDAB_3745169
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13554
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.

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