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验证数据展示

  • WB analysis of human heart using 22274-1-AP

    WB analysis of human heart using 22274-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 22274-1-AP (CALML6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22274-1-AP

    IHC staining of human heart using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 22274-1-AP

    IHC staining of human heart using 22274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 22274-1-AP (CALML6 antibody) at dilution of 1:50 (under 40x lens).

CALML6 Polyclonal antibody

CALML6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22274-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

Calmodulin like protein 6, calmodulin like 6, CALGP, Calglandulin-like protein, Calglandulin like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, pig heart tissue
Positive IHC detected inhuman heart tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

22274-1-AP targets CALML6 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, pig
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calmodulin-like 6
别名 Calmodulin like protein 6, calmodulin like 6, CALGP, Calglandulin-like protein, Calglandulin like protein
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号NM_138705
基因名称 CALML6
Gene ID (NCBI) 163688
RRIDAB_11182942
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TD86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CALML6, also named as CAGLP, CALGP , belongs to the calmodulin family and Calglandulin subfamily. It contains 4 EF-hand domains. The antibody is specific to CALML6.

实验方案

Product Specific Protocols
WB protocol for CALML6 antibody 22274-1-APDownload protocol
IHC protocol for CALML6 antibody 22274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Technol Cancer Res Treat

A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer

Authors - Linjing Li
View Article
humanIHC

Gene

CALML6 as a potential diagnostic marker for thyroid cancer promotes thyroid cancer cell proliferation via modulating the immune microenvironment

Authors - Caixia Cheng
View Article