• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

    WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    WB analysis of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    WB result of CALD1 antibody (66693-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CALD1 transfected HeLa cells. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALD1 antibody included
  • IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    IHC staining of human colon cancer using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALD1 antibody included
  • IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)

    IF Staining of HepG2 using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CALD1 antibody (66693-1-Ig, Clone: 1E1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    FC experiment of HeLa using 66693-1-Ig (same clone as 66693-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CALD1 (66693-1-Ig, Clone:1E1C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

    WB analysis using 66693-1-Ig (same clone as 66693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66693-1-Ig (CALD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

  • Sample test of MP51351-1

    Sample test of MP51351-1

    Sample test of MP51351-1, CALD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66693-1-PBS. Detection antibody: 66693-2-PBS.

  • IHC staining of human colon using 66693-1-Ig (same clone as 66693-1-PBS)

    IHC staining of human colon using 66693-1-Ig (same clone as 66693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66693-1-Ig (CALD1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66693-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CALD1 antibody included
  • Cytometric bead array standard curve of MP51351-1

    Cytometric bead array standard curve of MP51351-1

    Cytometric bead array standard curve of MP51351-1, CALD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66693-1-PBS. Detection antibody: 66693-2-PBS. Standard:Ag13676. Range: 0.391-400 ng/mL.

  • Featured Product
  • KD/KO验证

CALD1 Monoclonal antibody, PBS Only (Capture)

CALD1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test
Cat No. 66693-1-PBS

产品说明书

CloneNo. 1E1C11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test

别名

Caldesmon, 1E1C11, CAD, caldesmon 1, CDM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.

产品信息

66693-1-PBS targets CALD1 as part of a matched antibody pair:

MP51351-1: 66693-1-PBS capture and 66693-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 CALD1 fusion protein Ag13676 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 caldesmon 1
别名 Caldesmon, 1E1C11, CAD, caldesmon 1, CDM
计算分子量 538 aa, 63 kDa
观测分子量 70 kDa~75 kDa
GenBank蛋白编号BC040354
基因名称 CALD1
Gene ID (NCBI) 800
RRIDAB_2882047
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ05682
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C