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验证数据展示

  • WB analysis using 13464-1-AP (same clone as 13464-1-PBS)

    WB analysis using 13464-1-AP (same clone as 13464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13464-1-AP (CABLES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 13464-1-AP (same clone as 13464-1-PBS)

    IHC staining of human colon using 13464-1-AP (same clone as 13464-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CABLES1 antibody included
  • IF Staining of MCF-7 using 13464-1-AP (same clone as 13464-1-PBS)

    IF Staining of MCF-7 using 13464-1-AP (same clone as 13464-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

CABLES1 Polyclonal antibody, PBS Only

CABLES1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13464-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ik3-1, HsT2563, CABLES

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CABLES1 Ready-To-Use IHC Kit. CABLES1 primary antibody included.

产品信息

13464-1-PBS targets CABLES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4251

Product name: Recombinant human CABLES1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-368 aa of BC037218

Sequence: SQRSSLETLEDIEENAPLRRCRTLSGSPRPKNFKKIHFIKNMRQHDTRNGRIVLISGRRSFCSIFSVLPYRDSTQVGDLKLDGGRQSTGAVSLKEIIGLEGVELGADGKTVSYTQFLLPTNAFGARRNTIDSTSSFSQFRNLSHRSLSIGRASGTQGSLDTGSDLGDFMDYDPNLLDDPQWPCGKHKRVLIFPSYMTTVIDYVKPSDLKKDMNETFKEKFPHIKLTLSKIRSLKREMRKLAQEDCGLEEPTVAMAFVYFEKLALKGKLNKQNRKLCAGACVLLAAKIGSDLKKHEVKHLIDKLEEKFRLNRRELIAFEFPVLVALEFALHLPEHEVMPHYRRLVQSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cdk5 and Abl enzyme substrate 1
别名 Ik3-1, HsT2563, CABLES
计算分子量 568 aa, 63 kDa
观测分子量68 kDa, 42 kDa
GenBank蛋白编号BC037218
基因名称 CABLES1
Gene ID (NCBI) 91768
RRIDAB_3669163
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDN4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK5 and ABL1 enzyme substrate 1 (CABLES1) is a novel Cdk2, Cdk3, and Cdk5 binding protein, which acts as a link between the Cdks and nonreceptor tyrosine kinases and regulates the activity of Cdks by enhancing their Y15 phosphorylation. CABLES1 is a candidate tumor suppressor that negatively regulates cell growth by inhibiting cyclin-dependent kinases.

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