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验证数据展示

  • WB analysis using 13464-1-AP

    WB analysis using 13464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13464-1-AP (CABLES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 13464-1-AP

    IHC staining of human colon using 13464-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CABLES1 antibody included
  • IF Staining of MCF-7 using 13464-1-AP

    IF Staining of MCF-7 using 13464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CABLES1 Polyclonal antibody

CABLES1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13464-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Ik3-1, HsT2563, CABLES

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 205 cells, HT-29 cells, SH-SY5Y cells
Positive IHC detected inhuman colon tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CABLES1 Ready-To-Use IHC Kit. CABLES1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13464-1-AP targets CABLES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CABLES1 fusion protein Ag4251 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cdk5 and Abl enzyme substrate 1
别名 Ik3-1, HsT2563, CABLES
计算分子量 568 aa, 63 kDa
观测分子量68 kDa, 42 kDa
GenBank蛋白编号BC037218
基因名称 CABLES1
Gene ID (NCBI) 91768
RRIDAB_3669163
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDN4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK5 and ABL1 enzyme substrate 1 (CABLES1) is a novel Cdk2, Cdk3, and Cdk5 binding protein, which acts as a link between the Cdks and nonreceptor tyrosine kinases and regulates the activity of Cdks by enhancing their Y15 phosphorylation. CABLES1 is a candidate tumor suppressor that negatively regulates cell growth by inhibiting cyclin-dependent kinases.

实验方案

Product Specific Protocols
WB protocol for CABLES1 antibody 13464-1-APDownload protocol
IHC protocol for CABLES1 antibody 13464-1-APDownload protocol
IF protocol for CABLES1 antibody 13464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols