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验证数据展示

  • WB analysis of HeLa using 27797-1-AP

    WB analysis of HeLa using 27797-1-AP

    WB result of C9orf64 antibody (27797-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C9orf64 transfected HeLa cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27797-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27797-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27797-1-AP (C9orf64 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

C9orf64 Polyclonal antibody

C9orf64 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27797-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Queuosine-nucleotide N-glycosylase/hydrolase, Queuosine 5'-phosphate N-glycosylase/hydrolase, Queuine salvage protein QNG1, Q-nucleotide N-glycosylase 1, QNG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27797-1-AP targets C9orf64 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C9orf64 fusion protein Ag23069 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 64
别名 Queuosine-nucleotide N-glycosylase/hydrolase, Queuosine 5'-phosphate N-glycosylase/hydrolase, Queuine salvage protein QNG1, Q-nucleotide N-glycosylase 1, QNG1
计算分子量 341 aa, 39 kDa
观测分子量34-39 kDa
GenBank蛋白编号BC004407
基因名称 C9orf64
Gene ID (NCBI) 84267
RRIDAB_2918132
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T6V5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C9orf64 antibody 27797-1-APDownload protocol
IHC protocol for C9orf64 antibody 27797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols