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验证数据展示

  • WB analysis using 21012-1-AP (same clone as 21012-1-PBS)

    WB analysis using 21012-1-AP (same clone as 21012-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)

    IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

C9orf117 Polyclonal antibody, PBS Only

C9orf117 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21012-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21012-1-PBS targets C9orf117 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15234

Product name: Recombinant human C9orf117 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC141809

Sequence: MAPKKSVSKAGKELEVKKKGGKKEPVVAVEPPLAKEMKEFYHIQIRDLEDRLARYQRKWDELAVQEKMFRQEFEQLANNKKEIVAFLKRTLNQQVDEITDLNEQLQNLQLAKEMEKDAFEAQLAQVRHEFQETKDQLTTENIILGGKLAALEEFRLQKEEVTDKFTLLEEQVRKQENEFRDYAYNLEKKSVLDKDRLRKEIIQRVNLVANEFHKVTTNRMWETTKRAIKENNGITLQMARVSQQGMKLLQENEQLKGRQNNLCKQLELLENTQKVMARHKRGHQKIILMLTKKCQEQQQDTKEAEELRLLLSQLEQRSLQLQVDNQALKSQRDQLSLQLEQQQVDLQRLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 117
别名 AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin
计算分子量 520 aa, 61 kDa
观测分子量60 kDa
GenBank蛋白编号BC141809
基因名称 C9orf117
Gene ID (NCBI) 286207
RRIDAB_3669353
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JU67
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).

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