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验证数据展示

  • WB analysis using 21012-1-AP

    WB analysis using 21012-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21012-1-AP

    IHC staining of mouse brain using 21012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21012-1-AP

    IHC staining of mouse brain using 21012-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 21012-1-AP

    IF Staining of A549 using 21012-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C9orf117 Polyclonal antibody

C9orf117 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21012-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, Raji cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21012-1-AP targets C9orf117 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15234

Product name: Recombinant human C9orf117 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC141809

Sequence: MAPKKSVSKAGKELEVKKKGGKKEPVVAVEPPLAKEMKEFYHIQIRDLEDRLARYQRKWDELAVQEKMFRQEFEQLANNKKEIVAFLKRTLNQQVDEITDLNEQLQNLQLAKEMEKDAFEAQLAQVRHEFQETKDQLTTENIILGGKLAALEEFRLQKEEVTDKFTLLEEQVRKQENEFRDYAYNLEKKSVLDKDRLRKEIIQRVNLVANEFHKVTTNRMWETTKRAIKENNGITLQMARVSQQGMKLLQENEQLKGRQNNLCKQLELLENTQKVMARHKRGHQKIILMLTKKCQEQQQDTKEAEELRLLLSQLEQRSLQLQVDNQALKSQRDQLSLQLEQQQVDLQRLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 117
别名 AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin
计算分子量 520 aa, 61 kDa
观测分子量60 kDa
GenBank蛋白编号BC141809
基因名称 C9orf117
Gene ID (NCBI) 286207
RRIDAB_3669353
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JU67
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).

实验方案

Product Specific Protocols
IHC protocol for C9orf117 antibody 21012-1-APDownload protocol
WB protocol for C9orf117 antibody 21012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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