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验证数据展示

  • WB analysis of human plasma using 25184-1-AP (same clone as 25184-1-PBS)

    WB analysis of human plasma using 25184-1-AP (same clone as 25184-1-PBS)

    human plasma was subjected to SDS PAGE followed by western blot with 25184-1-AP (C8B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25184-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25184-1-AP (same clone as 25184-1-PBS)

    IHC staining of human liver using 25184-1-AP (same clone as 25184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25184-1-AP (C8B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25184-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25184-1-AP (same clone as 25184-1-PBS)

    IHC staining of human liver using 25184-1-AP (same clone as 25184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25184-1-AP (C8B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25184-1-PBS in a different storage buffer formulation.

C8B Polyclonal antibody, PBS Only

C8B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25184-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CO8B, Complement component 8 subunit beta, complement component 8, beta polypeptide, Complement component C8 beta chain

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25184-1-PBS targets C8B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18388

Product name: Recombinant human C8B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 242-591 aa of BC130575

Sequence: RNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 8, beta polypeptide
别名 CO8B, Complement component 8 subunit beta, complement component 8, beta polypeptide, Complement component C8 beta chain
计算分子量 591 aa, 67 kDa
观测分子量64 kDa
GenBank蛋白编号BC130575
基因名称 C8B
Gene ID (NCBI) 732
RRIDAB_3085774
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07358
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The beta chain of complement component 8 (C8B) is one of three different subunits (alpha, beta, and gamma) of the complement component 8 (C8), which is a component of the MAC (membrane attack complex). The deficiency of C8B leads to the type II of C8 deficiency, leading to the malfunction of MAC.

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