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验证数据展示

  • WB analysis of human plasma using 25184-1-AP

    WB analysis of human plasma using 25184-1-AP

    human plasma was subjected to SDS PAGE followed by western blot with 25184-1-AP (C8B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25184-1-AP

    IHC staining of human liver using 25184-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25184-1-AP (C8B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25184-1-AP

    IHC staining of human liver using 25184-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25184-1-AP (C8B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C8B Polyclonal antibody

C8B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25184-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25184-1-AP targets C8B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18388

Product name: Recombinant human C8B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 242-591 aa of BC130575

Sequence: RNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 8, beta polypeptide
别名
计算分子量 591 aa, 67 kDa
观测分子量64 kDa
GenBank蛋白编号BC130575
基因名称 C8B
Gene ID (NCBI) 732
RRIDAB_3085774
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07358
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The beta chain of complement component 8 (C8B) is one of three different subunits (alpha, beta, and gamma) of the complement component 8 (C8), which is a component of the MAC (membrane attack complex). The deficiency of C8B leads to the type II of C8 deficiency, leading to the malfunction of MAC.

实验方案

Product Specific Protocols
IHC protocol for C8B antibody 25184-1-APDownload protocol
WB protocol for C8B antibody 25184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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