验证数据展示
经过测试的应用
Positive WB detected in | A375 cells |
Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A375 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24953-1-AP targets C6orf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | C6orf15 fusion protein Ag20276 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | chromosome 6 open reading frame 15 |
别名 | UNQ1840/PRO3566, STG, Protein STG |
计算分子量 | 325 aa, 34 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC153157 |
基因名称 | C6orf15 |
Gene ID (NCBI) | 29113 |
RRID | AB_2879818 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6UXA7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.
实验方案
Product Specific Protocols | |
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WB protocol for C6orf15 antibody 24953-1-AP | Download protocol |
IHC protocol for C6orf15 antibody 24953-1-AP | Download protocol |
IF protocol for C6orf15 antibody 24953-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell Int C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer | ||
Pathol Res Pract C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer | ||
Ann Diagn Pathol Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein |