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C6orf15 Polyclonal antibody

C6orf15 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 24953-1-AP

Print datasheet

Synonyms

C6orf15, Protein STG, STG

验证数据展示

  • WB analysis using 24953-1-AP

    WB analysis using 24953-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 24953-1-AP (C6orf15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skin using 24953-1-AP

    IHC staining of mouse skin using 24953-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A375 using 24953-1-AP

    IF Staining of A375 using 24953-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using C6orf15 antibody (24953-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA375 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

24953-1-AP targets C6orf15 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C6orf15 fusion protein Ag20276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 6 open reading frame 15
Synonyms C6orf15, Protein STG, STG
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC153157
Gene Symbol C6orf15
Gene ID (NCBI) 29113
RRIDAB_2879818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.

实验方案

Product Specific Protocols
WB protocol for C6orf15 antibody 24953-1-APDownload protocol
IHC protocol for C6orf15 antibody 24953-1-APDownload protocol
IF protocol for C6orf15 antibody 24953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Pathol Res Pract

C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer

Authors - Xiaoyu Xiong
View Article