C6orf15 Polyclonal antibody

C6orf15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24953-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

UNQ1840/PRO3566, STG, Protein STG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA375 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24953-1-AP targets C6orf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 C6orf15 fusion protein Ag20276 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 15
别名 UNQ1840/PRO3566, STG, Protein STG
计算分子量 325 aa, 34 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC153157
基因名称 C6orf15
Gene ID (NCBI) 29113
RRIDAB_2879818
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXA7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.

实验方案

Product Specific Protocols
WB protocol for C6orf15 antibody 24953-1-APDownload protocol
IHC protocol for C6orf15 antibody 24953-1-APDownload protocol
IF protocol for C6orf15 antibody 24953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cancer Cell Int

C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer

Authors - Jiankang Yu
humanIHC

Pathol Res Pract

C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer

Authors - Xiaoyu Xiong
humanIHC

Ann Diagn Pathol

Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein

Authors - Leilei Liu
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