验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells |
| Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24953-1-AP targets C6orf15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20276 Product name: Recombinant human C6orf15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-325 aa of BC153157 Sequence: RSIGVVEEKVSQNLGTNLPQLGQPSSTGPSNSEHPQPALDPRSNDLARVPLKLSVPASDGFPPAGGSAVQRWPPSWGLPAMDSWPPEDPWQMMAAAAEDRLGEALPEELSYLSSAAALAPGSGPLPGESSPDATGLSPKASLLHQDSESRRLPRSNSLGAGGKILSQRPPWSLIHRVLPDHPWGTLNPSVSWGGGGPGTGWGTRPMPHPEGIWGINNQPPGTSWGNINRYPGGSWGNINRYPGGSWGNINRYPGGSWGNIHLYPGINNPFPPGVLRPPGSSWNIPAGFPNPPSPRLQWG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 15 |
| 别名 | Protein STG, STG, UNQ1840/PRO3566 |
| 计算分子量 | 325 aa, 34 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC153157 |
| 基因名称 | C6orf15 |
| Gene ID (NCBI) | 29113 |
| RRID | AB_2879818 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UXA7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| IHC protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| WB protocol for C6orf15 antibody 24953-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer | ||
Pathol Res Pract C6orf15 acts as a potential novel marker of adverse pathological features and prognosis for colon cancer | ||
Ann Diagn Pathol Differential diagnosis of gastric low- and high grade dysplasia using C6orf15 protein |
