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验证数据展示

  • WB analysis using 20444-1-AP

    WB analysis using 20444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20444-1-AP (C5orf44 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20444-1-AP

    IHC staining of human kidney using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 20444-1-AP

    IHC staining of human kidney using 20444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 20444-1-AP

    IF Staining of HEK-293 using 20444-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20444-1-AP (C5orf44 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 20444-1-AP

    IF Staining of HEK-293 using 20444-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf44 antibody (20444-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C5orf44 Polyclonal antibody

C5orf44 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20444-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

UPF0533 protein C5orf44, TRAPPC13, Trafficking protein particle complex subunit 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20444-1-AP targets C5orf44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C5orf44 fusion protein Ag14267 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 5 open reading frame 44
别名 UPF0533 protein C5orf44, TRAPPC13, Trafficking protein particle complex subunit 13
计算分子量 417 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC012006
基因名称 C5orf44
Gene ID (NCBI) 80006
RRIDAB_10697663
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5PLN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of C5orf44 has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 47 kDa, and this antibody specially recognises the 47 kDa protein.

实验方案

Product Specific Protocols
WB protocol for C5orf44 antibody 20444-1-APDownload protocol
IHC protocol for C5orf44 antibody 20444-1-APDownload protocol
IF protocol for C5orf44 antibody 20444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols