C4BPB Polyclonal antibody
C4BPB Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, HeLa cells, mouse ovary tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
15837-1-AP targets C4BPB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | C4BPB fusion protein Ag8587 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | complement component 4 binding protein, beta |
| Synonyms | C4b binding protein beta chain, C4BP, C4BPB |
| Calculated Molecular Weight | 251 aa, 28 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC005378 |
| Gene Symbol | C4BPB |
| Gene ID (NCBI) | 725 |
| RRID | AB_2067035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP beta chain (C4BPB).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C4BPB antibody 15837-1-AP | Download protocol |
| IHC protocol for C4BPB antibody 15837-1-AP | Download protocol |
| IF protocol for C4BPB antibody 15837-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
BMC Cancer The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer. | ||
World J Surg Oncol Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma |
