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验证数据展示

  • WB analysis of human plasma using 17160-1-AP (same clone as 17160-1-PBS)

    WB analysis of human plasma using 17160-1-AP (same clone as 17160-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

C4 Gamma Chain Polyclonal antibody, PBS Only

C4 Gamma Chain Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17160-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17160-1-PBS targets C4 Gamma Chain in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10449

Product name: Recombinant human C4A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-202 aa of BC012372

Sequence: EVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREGSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLESNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 4A (Rodgers blood group)
别名
计算分子量 1744 aa, 193 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC012372
基因名称 C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2923565
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0C0L4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4A protein recognizes the C4 gamma chain.

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