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验证数据展示

  • WB analysis of human plasma using 17160-1-AP

    WB analysis of human plasma using 17160-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17160-1-AP

    IHC staining of human liver cancer using 17160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17160-1-AP

    IHC staining of human liver cancer using 17160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C4 Gamma Chain Polyclonal antibody

C4 Gamma Chain Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17160-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Acidic complement C4, C4, C4 (gamma chain), C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17160-1-AP targets C4 Gamma Chain in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 C4 Gamma Chain fusion protein Ag10449 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 4A (Rodgers blood group)
别名 Acidic complement C4, C4, C4 (gamma chain), C4A, C4A2, C4A3, C4A4, C4A6, C4S, CO4, Complement C4 A, CPAMD2, RG
计算分子量 1744 aa, 193 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC012372
基因名称 C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2923565
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0C0L4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4A protein recognizes the C4 gamma chain.

实验方案

Product Specific Protocols
WB protocol for C4 Gamma Chain antibody 17160-1-APDownload protocol
IHC protocol for C4 Gamma Chain antibody 17160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols