验证数据展示
经过测试的应用
| Positive WB detected in | human plasma, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17160-1-AP targets C4 Gamma Chain in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10449 Product name: Recombinant human C4A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1543-1744 aa of NM_001252204 Sequence: EVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREGSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLESNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | complement component 4A (Rodgers blood group) |
| 别名 | C4 (gamma chain), C4A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4 |
| 计算分子量 | 1744 aa, 193 kDa |
| 观测分子量 | 190 kDa, 30-35 kDa |
| GenBank蛋白编号 | NM_001252204 |
| 基因名称 | C4 Gamma Chain |
| Gene ID (NCBI) | 720 |
| RRID | AB_2923565 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0C0L4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor that is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody, raised against 1543-1744aa of human C4A protein, recognizes the C4 gamma chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C4 Gamma Chain antibody 17160-1-AP | Download protocol |
| WB protocol for C4 Gamma Chain antibody 17160-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
