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验证数据展示

  • WB analysis using 32718-1-AP

    WB analysis using 32718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32718-1-AP (C3orf62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 32718-1-AP

    IHC staining of mouse testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 32718-1-AP

    IHC staining of rat testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C3orf62 Polyclonal antibody

C3orf62 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Uncharacterized protein C3orf62

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32718-1-AP targets C3orf62 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37728

Product name: Recombinant human C3orf62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-267 aa of BC023586

Sequence: MHYIKTWSLLGEMSEKLRRCRKELTAAIDRAFEGVSYSQECTGQQRLELSAAPLSFSLPVHRLLCRRHPLAACSSAAPFAAVPCAPENENPAFATNHAPVNAKPHALCPERKPLTSKENVLMHSSILAPERESWRTAGEGENWRKENLRKDMERDLKADSNMPLNNSSQEVTKDLLDMIDHTSIRTIEELAGKIEFENELNHMCGHCQDSPFKEEAWALLMDKSPQKATDADPGSLKQAFDDHNIVETVLDLEEDYNVMTSFKYQIE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 3 open reading frame 62
别名 Uncharacterized protein C3orf62
计算分子量30 kDa
观测分子量38 kDa
GenBank蛋白编号BC023586
基因名称 C3orf62
Gene ID (NCBI) 375341
RRIDAB_3742721
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZUJ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arrest-type spermatogenic failure refers to the arrest of spermatogenesis at a specific stage, such as spermatogonia, spermatocytes, or spermatids. It is mainly caused by genetic factors, particularly variants in key spermatogenic genes (e.g., TEX11, MSH5, C3ORF62, MEI1, etc.), and represents one of the most distinctive forms of male infertility (PMID: 41669890). The C3orf62 is essential for normal spermatogenesis and male fertility (PMID: 33064489; 38175751).

实验方案

Product Specific Protocols
IHC protocol for C3orf62 antibody 32718-1-APDownload protocol
WB protocol for C3orf62 antibody 32718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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