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验证数据展示

  • WB analysis using 29313-1-AP

    WB analysis using 29313-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29313-1-AP (C22orf32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 29313-1-AP

    IF Staining of A431 using 29313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C22orf32 antibody (29313-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

C22orf32 Polyclonal antibody

C22orf32 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29313-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SMDT1, EMRE, DDDD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSW480 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29313-1-AP targets C22orf32 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C22orf32 fusion protein Ag29162 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 22 open reading frame 32
别名 SMDT1, EMRE, DDDD
观测分子量10 kDa
GenBank蛋白编号BC024237
基因名称 C22orf32
Gene ID (NCBI) 91689
RRIDAB_3669681
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4I9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C22orf32, also known as SMDT1 or EMRE, is a 10 kDa single-channel transmembrane that contains a mitochondrial targeting sequence, a transmembrane region, and a conserved aspart-rich C-terminal. It acts as a subunit of the MCU complex and increases mCa2+ uptake by activating the MCU function (PMID: 38417574).

实验方案

Product Specific Protocols
WB protocol for C22orf32 antibody 29313-1-APDownload protocol
IF protocol for C22orf32 antibody 29313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols