验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human brain tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21466-1-AP targets TMEM230 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TMEM230 fusion protein Ag15080 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 20 open reading frame 30 |
| 别名 | C20orf30, HSPC274, TMEM230, UNQ2432/PRO4992 |
| 计算分子量 | 183 aa, 20 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC011990 |
| 基因名称 | TMEM230 |
| Gene ID (NCBI) | 29058 |
| RRID | AB_10791969 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96A57 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TMEM230 antibody 21466-1-AP | Download protocol |
| IHC protocol for TMEM230 antibody 21466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins. | ||
J Alzheimers Dis TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease | ||
Exp Anim TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models | ||
Int J Mol Sci Transmembrane Protein TMEM230, Regulator of Glial Cell Vascular Mimicry and Endothelial Cell Angiogenesis in High-Grade Heterogeneous Infiltrating Gliomas and Glioblastoma |
