验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HSC-T6 cells, rabbit liver tissue, HepG2 cells, LNCaP cells, NIH/3T3 cells, human placenta tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67464-1-Ig targets C20orf3/APMAP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, rabbit |
| 文献引用反应性 | rabbit |
| 免疫原 |
CatNo: Ag29996 Product name: Recombinant human C20orf3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 66-416 aa of BC003501 Sequence: DPQPLSFKEPPLLLGVLHPNTKLRQAERLFENQLVGPESIAHIGDVMFTGTADGRVVKLENGEIETIARFGSGPCKTRDDEPVCGRPLGIRAGPNGTLFVADAYKGLFEVNPWKREVKLLLSSETPIEGKNMSFVNDLTVTQDGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVKVLLDQLRFPNGVQLSPAEDFVLVAETTMARIRRVYVSGLMKGGADLFVENMPGFPDNIRPSSSGGYWVGMSTIRPNPGFSMLDFLSERPWIKRMIFKLFSQETVMKFVPRYSLVLELSDSGAFRRSLHDPDGLVATYISEVHEHDGHLYLGSFRSPFLCRLSLQAV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 20 open reading frame 3 |
| 别名 | C20orf3, 1C6C2, Adipocyte plasma membrane-associated protein, APMAP, BSCv |
| 计算分子量 | 416 aa, 46 kDa |
| 观测分子量 | 42-46 kDa |
| GenBank蛋白编号 | BC003501 |
| 基因名称 | APMAP |
| Gene ID (NCBI) | 57136 |
| RRID | AB_2882693 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9HDC9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for C20orf3/APMAP antibody 67464-1-Ig | Download protocol |
| WB protocol for C20orf3/APMAP antibody 67464-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurochem Integrative Analysis of DNA Methylation and Gene Expression Reveals Key Molecular Signatures in Spatial Memory Impairment of Sepsis-Associated Encephalopathy | ||
Food Chem (Oxf) Transcriptomics and 4D-DIA proteomic analysis reveal differences in meat quality between Sichuan white rabbits and New Zealand white rabbits. |
