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验证数据展示

  • WB analysis using 67464-1-Ig

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67464-1-Ig

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67464-1-Ig

    WB analysis of HepG2 using 67464-1-Ig

    WB result of C20orf3/APMAP antibody (67464-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C20orf3/APMAP transfected HepG2 cells.

  • IHC staining of human prostate cancer using 67464-1-Ig

    IHC staining of human prostate cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67464-1-Ig

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 67464-1-Ig

    IHC staining of human prostate cancer using 67464-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67464-1-Ig

    WB analysis using 67464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67464-1-Ig (C20orf3/APMAP antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

C20orf3/APMAP Monoclonal antibody

C20orf3/APMAP Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67464-1-Ig

产品说明书

CloneNo. 1C6C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, ELISA

别名

C20orf3, 1C6C2, Adipocyte plasma membrane-associated protein, APMAP, BSCv

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HSC-T6 cells, rabbit liver tissue, HepG2 cells, LNCaP cells, NIH/3T3 cells, human placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67464-1-Ig targets C20orf3/APMAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag29996

Product name: Recombinant human C20orf3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 66-416 aa of BC003501

Sequence: DPQPLSFKEPPLLLGVLHPNTKLRQAERLFENQLVGPESIAHIGDVMFTGTADGRVVKLENGEIETIARFGSGPCKTRDDEPVCGRPLGIRAGPNGTLFVADAYKGLFEVNPWKREVKLLLSSETPIEGKNMSFVNDLTVTQDGRKIYFTDSSSKWQRRDYLLLVMEGTDDGRLLEYDTVTREVKVLLDQLRFPNGVQLSPAEDFVLVAETTMARIRRVYVSGLMKGGADLFVENMPGFPDNIRPSSSGGYWVGMSTIRPNPGFSMLDFLSERPWIKRMIFKLFSQETVMKFVPRYSLVLELSDSGAFRRSLHDPDGLVATYISEVHEHDGHLYLGSFRSPFLCRLSLQAV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 3
别名 C20orf3, 1C6C2, Adipocyte plasma membrane-associated protein, APMAP, BSCv
计算分子量 416 aa, 46 kDa
观测分子量 42-46 kDa
GenBank蛋白编号BC003501
基因名称 APMAP
Gene ID (NCBI) 57136
RRIDAB_2882693
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9HDC9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for C20orf3/APMAP antibody 67464-1-IgDownload protocol
WB protocol for C20orf3/APMAP antibody 67464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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