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验证数据展示

  • IF Staining of HEK-293 using 83626-3-RR

    IF Staining of HEK-293 using 83626-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83626-3-RR

    FC experiment of U2OS using 83626-3-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of mouse cerebellum using 83626-3-RR

    IHC staining of mouse cerebellum using 83626-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 83626-3-RR

    IHC staining of mouse cerebellum using 83626-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83626-3-RR

    Affinity and Kinetic Characterization of 83626-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Recombinant antibody

C1orf83 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83626-3-RR

产品说明书

CloneNo. 240678D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83626-3-RR targets C1orf83 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 C1orf83 fusion protein Ag15040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 83
别名 Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112
计算分子量 208 aa, 23 kDa
GenBank蛋白编号BC035374
基因名称 C1orf83
Gene ID (NCBI) 127428
RRIDAB_3671238
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96MN5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf83, also known as TCEANC2, and the rs10788972 variant of the TCEANC2 gene in the PARK10 region were identified as being strongly associated with sporadic PD in the US population.

实验方案

Product Specific Protocols
IHC protocol for C1orf83 antibody 83626-3-RRDownload protocol
IF protocol for C1orf83 antibody 83626-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols