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验证数据展示

  • IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

    IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

    FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00578-2

    Cytometric bead array standard curve of MP00578-2

    Cytometric bead array standard curve of MP00578-2, C1orf83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83626-1-PBS. Detection antibody: 83626-3-PBS. Standard: Ag15040. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83626-3-RR

    Affinity and Kinetic Characterization of 83626-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Recombinant antibody, PBS Only (Detector)

C1orf83 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83626-3-PBS

产品说明书

CloneNo. 240678D10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83626-3-PBS targets C1orf83 as part of a matched antibody pair:

MP00578-2: 83626-1-PBS capture and 83626-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 C1orf83 fusion protein Ag15040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 1 open reading frame 83
别名 Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112
计算分子量 208 aa, 23 kDa
GenBank蛋白编号BC035374
基因名称 C1orf83
Gene ID (NCBI) 127428
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96MN5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C