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验证数据展示

  • WB analysis of mouse testis using 25433-1-AP

    WB analysis of mouse testis using 25433-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25433-1-AP (C1orf56 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25433-1-AP

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 25433-1-AP

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 40x lens).

C1orf56 Polyclonal antibody

C1orf56 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25433-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

UNQ547/PRO1104, RP11 316M1.9, Protein MENT, Methylated in normal thymocytes protein, MENT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25433-1-AP targets C1orf56 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 C1orf56 fusion protein Ag13860 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 56
别名 UNQ547/PRO1104, RP11 316M1.9, Protein MENT, Methylated in normal thymocytes protein, MENT
计算分子量 341 aa, 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC002469
基因名称 C1orf56
Gene ID (NCBI) 54964
RRIDAB_2880077
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUN1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf56, also named as MENT, is involved in control of cellular proliferation. It is extreme expressed in several cancers especially in neuroblastoma. We detected the endogenous protein 35 kDa in mouse testis.

实验方案

Product Specific Protocols
WB protocol for C1orf56 antibody 25433-1-APDownload protocol
IHC protocol for C1orf56 antibody 25433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.

Authors - Chenghao Zhanghuang
View Article