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C1orf54 Polyclonal antibody

C1orf54 Polyclonal Antibody for IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30247-1-AP

Print datasheet

Synonyms

C1orf54, FLJ23221

验证数据展示

  • IHC staining of mouse kidney using 30247-1-AP

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30247-1-AP

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 30247-1-AP

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 30247-1-AP

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30247-1-AP targets C1orf54 in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, IHC, ELISA
Tested Reactivity Human, mouse
Immunogen C1orf54 fusion protein Ag32876 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 54
Synonyms C1orf54, FLJ23221
GenBank Accession NumberBC017761
Gene Symbol C1orf54
Gene ID (NCBI) 79630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf54 is a newly identified protein encoded by an open reading frame on chromosome 1. C1orf54 is highly expressed in healthy mouse kidneys and is only expressed in tubular epithelial cells (TECs), not in glomeruli(PMID: 29999589). C1orf54 promotes renal repair and TEC proliferation through PI3K/AKT signalling, which alleviates kidney damage after ischaemia‐reperfusion injury (IRI) (PMID: 29999589).

实验方案

Product Specific Protocols
IHC protocol for C1orf54 antibody 30247-1-APDownload protocol
IF protocol for C1orf54 antibody 30247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols