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验证数据展示

  • IHC staining of mouse kidney using 30247-1-AP

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30247-1-AP

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 30247-1-AP

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 30247-1-AP

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C1orf54 Polyclonal antibody

C1orf54 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30247-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, IF-P, ELISA

别名

C1orf54, FLJ23221

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30247-1-AP targets C1orf54 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse
免疫原 C1orf54 fusion protein Ag32876 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 54
别名 C1orf54, FLJ23221
GenBank蛋白编号BC017761
基因名称 C1orf54
Gene ID (NCBI) 79630
RRIDAB_3086279
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf54 is a newly identified protein encoded by an open reading frame on chromosome 1. C1orf54 is highly expressed in healthy mouse kidneys and is only expressed in tubular epithelial cells (TECs), not in glomeruli(PMID: 29999589). C1orf54 promotes renal repair and TEC proliferation through PI3K/AKT signalling, which alleviates kidney damage after ischaemia‐reperfusion injury (IRI) (PMID: 29999589).

实验方案

Product Specific Protocols
IHC protocol for C1orf54 antibody 30247-1-APDownload protocol
IF protocol for C1orf54 antibody 30247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols