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C1orf106 Polyclonal antibody

C1orf106 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21506-1-AP

Print datasheet

Synonyms

C1orf106

验证数据展示

  • WB analysis using 21506-1-AP

    WB analysis using 21506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 21506-1-AP

    IHC staining of mouse kidney using 21506-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 21506-1-AP

    IHC staining of mouse kidney using 21506-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 21506-1-AP

    IF Staining of HEK-293 using 21506-1-AP

    Immunofluorescent analysis of HEK-293 cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse kidney using 21506-1-AP

    IP experiment of mouse kidney using 21506-1-AP

    IP result of anti-C1orf106 (IP:21506-1-AP, 4ug; Detection:21506-1-AP 1:500) with mouse kidney tissue lysate 4800ug.

经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21506-1-AP targets C1orf106 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen C1orf106 fusion protein Ag15987 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 1 open reading frame 106
Synonyms C1orf106
Calculated Molecular Weight 663 aa, 73 kDa
Observed Molecular Weight 70-73 kDa
GenBank Accession NumberBC106877
Gene Symbol C1orf106
Gene ID (NCBI) 55765
RRIDAB_10734431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KP66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf106 (also known as innate immunity activator, INAVA) has thus far been considered to play a significant role in intestinal inflammatory diseases. C1orf106 is highly expressed in the human intestine and intestinal epithelial cell lines but expressed at low levels in myeloid cells and mouse bone marrow-derived macrophages (PMID: 29420262). Recent studies have also shown that C1orf106 protects against colitis by stabilizing E-cadherin and bridges mucosal barrier function with inflammatory signaling (PMID: 31376015).

实验方案

Product Specific Protocols
WB protocol for C1orf106 antibody 21506-1-APDownload protocol
IHC protocol for C1orf106 antibody 21506-1-APDownload protocol
IF protocol for C1orf106 antibody 21506-1-APDownload protocol
IP protocol for C1orf106 antibody 21506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols