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验证数据展示

  • WB analysis using 21506-1-AP

    WB analysis using 21506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 21506-1-AP

    IHC staining of mouse kidney using 21506-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 21506-1-AP

    IHC staining of mouse kidney using 21506-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 21506-1-AP

    IF Staining of HEK-293 using 21506-1-AP

    Immunofluorescent analysis of HEK-293 cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse kidney using 21506-1-AP

    IP experiment of mouse kidney using 21506-1-AP

    IP result of anti-C1orf106 (IP:21506-1-AP, 4ug; Detection:21506-1-AP 1:500) with mouse kidney tissue lysate 4800ug.

C1orf106 Polyclonal antibody

C1orf106 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21506-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Innate immunity activator protein, INAVA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21506-1-AP targets C1orf106 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C1orf106 fusion protein Ag15987 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 1 open reading frame 106
别名 Innate immunity activator protein, INAVA
计算分子量 663 aa, 73 kDa
观测分子量 70-73 kDa
GenBank蛋白编号BC106877
基因名称 C1orf106
Gene ID (NCBI) 55765
RRIDAB_10734431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KP66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1orf106 (also known as innate immunity activator, INAVA) has thus far been considered to play a significant role in intestinal inflammatory diseases. C1orf106 is highly expressed in the human intestine and intestinal epithelial cell lines but expressed at low levels in myeloid cells and mouse bone marrow-derived macrophages (PMID: 29420262). Recent studies have also shown that C1orf106 protects against colitis by stabilizing E-cadherin and bridges mucosal barrier function with inflammatory signaling (PMID: 31376015).

实验方案

Product Specific Protocols
WB protocol for C1orf106 antibody 21506-1-APDownload protocol
IHC protocol for C1orf106 antibody 21506-1-APDownload protocol
IF protocol for C1orf106 antibody 21506-1-APDownload protocol
IP protocol for C1orf106 antibody 21506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols