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验证数据展示

  • WB analysis using 14554-1-AP (same clone as 14554-1-PBS)

    WB analysis using 14554-1-AP (same clone as 14554-1-PBS)

    rat plasma lysates were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C1S antibody included
  • IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C1S antibody included

C1S Polyclonal antibody, PBS Only

C1S Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14554-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy C1S Ready-To-Use IHC Kit. C1S primary antibody included.

产品信息

14554-1-PBS targets C1S in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5977

Product name: Recombinant human C1S protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-688 aa of BC056903

Sequence: DDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, s subcomponent
别名
计算分子量 77 kDa
观测分子量 88 kDa
GenBank蛋白编号BC056903
基因名称 C1S
Gene ID (NCBI) 716
RRIDAB_2243610
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09871
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

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