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验证数据展示

  • WB analysis using 32206-1-AP

    WB analysis using 32206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32206-1-AP (C1QL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 32206-1-AP

    IHC staining of rat brain using 32206-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32206-1-AP (C1QL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 32206-1-AP

    IHC staining of mouse brain using 32206-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32206-1-AP (C1QL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C1QL2 Polyclonal antibody

C1QL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32206-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C1q and tumor necrosis factor-related protein 10, C1q/TNF-related protein 10, Complement C1q-like protein 2, CTRP10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32206-1-AP targets C1QL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C1QL2 fusion protein Ag37614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component 1, q subcomponent-like 2
别名 C1q and tumor necrosis factor-related protein 10, C1q/TNF-related protein 10, Complement C1q-like protein 2, CTRP10
计算分子量29 kDa
观测分子量35 kDa
GenBank蛋白编号NM_182528
基因名称 C1QL2
Gene ID (NCBI) 165257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z5L3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement C1q-like protein 2 (C1QL2, also known as CTRP10) is a member of the C1q-like protein family. It is a secreted protein that plays a role in synaptic organization and function. C1QL2 is involved in the formation and maintenance of synapses, particularly in the central nervous system (CNS) (PMID: 20646056). It interacts with specific neurexin-3 isoforms to recruit synaptic vesicles and modulate synaptic transmission. Additionally, C1QL2 has been implicated in the regulation of hippocampal mossy fiber-CA3 synapse function, suggesting its role in synaptic plasticity and memory formation (PMID: 28219683).

实验方案

Product Specific Protocols
WB protocol for C1QL2 antibody 32206-1-APDownload protocol
IHC protocol for C1QL2 antibody 32206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols